Ontology highlight
ABSTRACT:
SUBMITTER: Allen WJ
PROVIDER: S-EPMC4907695 | biostudies-literature | 2016 May
REPOSITORIES: biostudies-literature
Allen William John WJ Corey Robin Adam RA Oatley Peter P Sessions Richard Barry RB Baldwin Steve A SA Radford Sheena E SE Tuma Roman R Collinson Ian I
eLife 20160516
The essential process of protein secretion is achieved by the ubiquitous Sec machinery. In prokaryotes, the drive for translocation comes from ATP hydrolysis by the cytosolic motor-protein SecA, in concert with the proton motive force (PMF). However, the mechanism through which ATP hydrolysis by SecA is coupled to directional movement through SecYEG is unclear. Here, we combine all-atom molecular dynamics (MD) simulations with single molecule FRET and biochemical assays. We show that ATP binding ...[more]