Ontology highlight
ABSTRACT:
SUBMITTER: Goyet E
PROVIDER: S-EPMC4908377 | biostudies-literature | 2016 Jun
REPOSITORIES: biostudies-literature
Goyet Elise E Bouquier Nathalie N Ollendorff Vincent V Perroy Julie J
Scientific reports 20160615
Resonance Energy Transfer (RET)-based technologies are used to report protein-protein interactions in living cells. Among them, Bioluminescence-initiated RET (BRET) provides excellent sensitivity but the low light intensity intrinsic to the bioluminescent process hampers its use for the localization of protein complexes at the sub-cellular level. Herein we have characterized the methodological conditions required to reliably perform single-cell BRET imaging using an extremely bright luciferase, ...[more]