Ontology highlight
ABSTRACT:
SUBMITTER: Pao KC
PROVIDER: S-EPMC4909137 | biostudies-literature | 2016 May
REPOSITORIES: biostudies-literature
Pao Kuan-Chuan KC Stanley Mathew M Han Cong C Lai Yu-Chiang YC Murphy Paul P Balk Kristin K Wood Nicola T NT Corti Olga O Corvol Jean-Christophe JC Muqit Miratul M K MM Virdee Satpal S
Nature chemical biology 20160307 5
E3 ligases represent an important class of enzymes, yet there are currently no chemical probes for profiling their activity. We develop a new class of activity-based probe by re-engineering a ubiquitin-charged E2 conjugating enzyme and demonstrate the utility of these probes by profiling the transthiolation activity of the RING-in-between-RING (RBR) E3 ligase parkin in vitro and in cellular extracts. Our study provides valuable insight into the roles, and cellular hierarchy, of distinct phosphor ...[more]