Ontology highlight
ABSTRACT:
SUBMITTER: Garrity AG
PROVIDER: S-EPMC4909396 | biostudies-literature | 2016 May
REPOSITORIES: biostudies-literature
Garrity Abigail G AG Wang Wuyang W Collier Crystal Md CM Levey Sara A SA Gao Qiong Q Xu Haoxing H
eLife 20160523
Impaired homeostasis of lysosomal Ca(2+) causes lysosome dysfunction and lysosomal storage diseases (LSDs), but the mechanisms by which lysosomes acquire and refill Ca(2+) are not known. We developed a physiological assay to monitor lysosomal Ca(2+) store refilling using specific activators of lysosomal Ca(2+) channels to repeatedly induce lysosomal Ca(2+) release. In contrast to the prevailing view that lysosomal acidification drives Ca(2+) into the lysosome, inhibiting the V-ATPase H(+) pump d ...[more]