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A novel bispecific antibody, BiSS, with potent anti-cancer activities.

ABSTRACT: One of the most active fields in cancer immunotherapy is the study of bispecific antibodies, which engage immune cells to kill cancer cells. However, a variety of issues are associated with most of current bispecific antibody formats. In this study, we present a novel bispecific antibody, BiSS (Bispecific antibody with Single domain, Single domain antibodies), which was constructed by linking 2 single domain antibodies, anti-CEA and anti-CD16, in tandem. Unlike most other bispecific antibodies, the BiSS antibody can be expressed and purified from E.coli in large quantities. By recruiting natural killer cells (NK cells) to CEA-positive cancer cells, BiSS led to cancer cell death in vitro. In xenograft models, the BiSS protein blocked cancer progression. The data suggested that the single domain-based bispecific antibody BiSS was functional and can be potentially applied to a broad range of immunotherapies.

SUBMITTER: Dong B

PROVIDER: S-EPMC4910920 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature

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A novel bispecific antibody, BiSS, with potent anti-cancer activities.

Dong Bin B Zhou Changhua C He Ping P Li Jing J Chen Siqi S Miao Ji J Li Qing Q Wang Zhong Z

Cancer biology & therapy 20160201 4

One of the most active fields in cancer immunotherapy is the study of bispecific antibodies, which engage immune cells to kill cancer cells. However, a variety of issues are associated with most of current bispecific antibody formats. In this study, we present a novel bispecific antibody, BiSS (Bispecific antibody with Single domain, Single domain antibodies), which was constructed by linking 2 single domain antibodies, anti-CEA and anti-CD16, in tandem. Unlike most other bispecific antibodies, ...[more]

PMID: 26828900

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| S-EPMC8438958 | biostudies-literature

A Novel Anti-B7-H3 × Anti-CD3 Bispecific Antibody with Potent Antitumor Activity.

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| S-EPMC8879513 | biostudies-literature

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| S-EPMC6694677 | biostudies-literature

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| S-EPMC9347186 | biostudies-literature

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Project description: Not available

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| S-EPMC7927792 | biostudies-literature

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