Unknown

Dataset Information

0

Pulmonary Th17 Antifungal Immunity Is Regulated by the Gut Microbiome.


ABSTRACT: Commensal microbiota are critical for the development of local immune responses. In this article, we show that gut microbiota can regulate CD4 T cell polarization during pulmonary fungal infections. Vancomycin drinking water significantly decreased lung Th17 cell numbers during acute infection, demonstrating that Gram-positive commensals contribute to systemic inflammation. We next tested a role for RegIII?, an IL-22-inducible antimicrobial protein with specificity for Gram-positive bacteria. Following infection, increased accumulation of Th17 cells in the lungs of RegIII?(-/-) and Il22(-/-) mice was associated with intestinal segmented filamentous bacteria (SFB) colonization. Although gastrointestinal delivery of rRegIII? decreased lung inflammatory gene expression and protected Il22(-/-) mice from weight loss during infection, it had no direct effect on SFB colonization, fungal clearance, or lung Th17 immunity. We further show that vancomycin only decreased lung IL-17 production in mice colonized with SFB. To determine the link between gut microbiota and lung immunity, serum-transfer experiments revealed that IL-1R ligands increase the accumulation of lung Th17 cells. These data suggest that intestinal microbiota, including SFB, can regulate pulmonary adaptive immune responses.

SUBMITTER: McAleer JP 

PROVIDER: S-EPMC4912941 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature

altmetric image

Publications


Commensal microbiota are critical for the development of local immune responses. In this article, we show that gut microbiota can regulate CD4 T cell polarization during pulmonary fungal infections. Vancomycin drinking water significantly decreased lung Th17 cell numbers during acute infection, demonstrating that Gram-positive commensals contribute to systemic inflammation. We next tested a role for RegIIIγ, an IL-22-inducible antimicrobial protein with specificity for Gram-positive bacteria. Fo  ...[more]

Similar Datasets

| S-EPMC9375732 | biostudies-literature
| S-SCDT-EMM-2019-11592 | biostudies-other
| S-EPMC3920589 | biostudies-other
| S-EPMC7293577 | biostudies-literature
| S-EPMC7411566 | biostudies-literature
| S-EPMC7067661 | biostudies-literature
| S-EPMC6456665 | biostudies-literature
| S-EPMC6750738 | biostudies-literature
| S-EPMC8665886 | biostudies-literature
| S-EPMC8456614 | biostudies-literature