Dataset Information

Age-related alterations in blood and colonic dendritic cell properties.

ABSTRACT:

Background

Dendritic cells (DC) determine initiation, type and location of immune responses and, in adults, show decreased Toll-like receptors and some increased cytokine levels on ageing. Few studies in children have characterised DC or explored DC-related mechanisms producing age-related immune changes.

Results

The pDC marker BDCA2 (but not CD123) was absent in pre-pubertal children and numbers of pDC decreased with age. Blood and colonic DC were more mature and activated in adults. Decrease in pDC numbers correlated with reduced GM-CSF levels with aging, but increasing IL-4 and IL-8 levels correlated with a more activated DC profile in blood. CXCL16 levels decreased with age.

Methods

Blood and colonic DC phenotypes were determined in healthy adults and children by flow cytometry and correlated with aging. Blood DC were divided into plasmacytoid (pDC) and myeloid (mDC) while only mDC were identified in colon. Serum cytokine levels were determined by multiplex cytokine assays and correlated with DC properties.

Conclusions

In children, lack of BDCA2, a receptor mediating antigen capture and inhibiting interferon induction, may be immunologically beneficial during immune development. Conversely, reduced pDC numbers, probably secondary to decreasing GM-CSF and increasing cytokine-induced maturation of DC are likely to determine deteriorating immunity with ageing.

SUBMITTER: Vora R

PROVIDER: S-EPMC4914258 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Age-related alterations in blood and colonic dendritic cell properties.

Vora Rakesh R Bernardo David D Durant Lydia L Reddi Durga D Hart Ailsa L AL Fell John M E JM Al-Hassi Hafid O HO Knight Stella C SC

Oncotarget 20160301 11

<h4>Background</h4>Dendritic cells (DC) determine initiation, type and location of immune responses and, in adults, show decreased Toll-like receptors and some increased cytokine levels on ageing. Few studies in children have characterised DC or explored DC-related mechanisms producing age-related immune changes.<h4>Results</h4>The pDC marker BDCA2 (but not CD123) was absent in pre-pubertal children and numbers of pDC decreased with age. Blood and colonic DC were more mature and activated in adu ...[more]

PMID: 26942871

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Dataset Information

Age-related alterations in blood and colonic dendritic cell properties.

Background

Results

Methods

Conclusions

Publications

Age-related alterations in blood and colonic dendritic cell properties.

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