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Dissecting AI-2-mediated quorum sensing through C5-analogue synthesis and biochemical analysis.

ABSTRACT: Autoinducer-2 (AI-2)-mediated quorum sensing (QS) is utilised for both intra- and inter-species communication by a wide variety of bacteria. An understanding of the mechanism of this communication has the potential to elucidate new targets for antibacterial therapeutics. Herein, we report the synthesis of DPD analogues with modified dynamic equilibria and the evaluation of their behaviour in Gram-negative bacteria. None of the compounds showed modulation of QS in S. Typhimurium, and although no antagonism of V. harveyi was observed, chloro-analogue C5-Cl-DPD showed modest agonism in this marine bacterium. This raises the possibility that access to a cyclic form of DPD may not be required for AI-2-mediated QS in V. harveyi.

SUBMITTER: Collins KC

PROVIDER: S-EPMC4915392 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature

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Dissecting AI-2-mediated quorum sensing through C5-analogue synthesis and biochemical analysis.

Collins Karen C KC Tsuchikama Kyoji K Lowery Colin A CA Zhu Jie J Janda Kim D KD

Tetrahedron 20150601 25

Autoinducer-2 (AI-2)-mediated quorum sensing (QS) is utilised for both intra- and inter-species communication by a wide variety of bacteria. An understanding of the mechanism of this communication has the potential to elucidate new targets for antibacterial therapeutics. Herein, we report the synthesis of DPD analogues with modified dynamic equilibria and the evaluation of their behaviour in Gram-negative bacteria. None of the compounds showed modulation of QS in <i>S.</i> Typhimurium, and altho ...[more]

PMID: 27340303

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