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Consensus protein design.

ABSTRACT: A popular and successful strategy in semi-rational design of protein stability is the use of evolutionary information encapsulated in homologous protein sequences. Consensus design is based on the hypothesis that at a given position, the respective consensus amino acid contributes more than average to the stability of the protein than non-conserved amino acids. Here, we review the consensus design approach, its theoretical underpinnings, successes, limitations and challenges, as well as providing a detailed guide to its application in protein engineering.

SUBMITTER: Porebski BT

PROVIDER: S-EPMC4917058 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature

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Consensus protein design.

Porebski Benjamin T BT Buckle Ashley M AM

Protein engineering, design & selection : PEDS 20160605 7

A popular and successful strategy in semi-rational design of protein stability is the use of evolutionary information encapsulated in homologous protein sequences. Consensus design is based on the hypothesis that at a given position, the respective consensus amino acid contributes more than average to the stability of the protein than non-conserved amino acids. Here, we review the consensus design approach, its theoretical underpinnings, successes, limitations and challenges, as well as providin ...[more]

PMID: 27274091

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