Ontology highlight
ABSTRACT:
SUBMITTER: Tang JC
PROVIDER: S-EPMC4922844 | biostudies-literature | 2016 May
REPOSITORIES: biostudies-literature
Tang Jonathan Cy JC Drokhlyansky Eugene E Etemad Behzad B Rudolph Stephanie S Guo Binggege B Wang Sui S Ellis Emily G EG Li Jonathan Z JZ Cepko Constance L CL
eLife 20160520
The ability to detect and/or manipulate specific cell populations based upon the presence of intracellular protein epitopes would enable many types of studies and applications. Protein binders such as nanobodies (Nbs) can target untagged proteins (antigens) in the intracellular environment. However, genetically expressed protein binders are stable regardless of antigen expression, complicating their use for applications that require cell-specificity. Here, we created a conditional system in whic ...[more]