Ontology highlight
ABSTRACT:
SUBMITTER: Huang Y
PROVIDER: S-EPMC4924517 | biostudies-literature | 2016 Jun
REPOSITORIES: biostudies-literature
Huang Yun Y Ferrie John J JJ Chen Xing X Zhang Yitao Y Szantai-Kis D Miklos DM Chenoweth David M DM Petersson E James EJ
Chemical communications (Cambridge, England) 20160601 50
Thioamide residues can be effective, minimally-perturbing fluorescence quenching probes for studying protein folding and proteolysis. In order to increase the level of quenching, we have here explored the use of adjacent dithioamides. We have found that they are more effective fluorescence quenchers, as expected, but we have also observed unexpected changes in the thioamide absorption spectra that may arise from n-to-π* interactions of the thiocarbonyls. We have made use of the increased quenchi ...[more]