Unknown

Dataset Information

0

15N Hyperpolarization of Imidazole-15N2 for Magnetic Resonance pH Sensing via SABRE-SHEATH.


ABSTRACT: 15N nuclear spins of imidazole-15N2 were hyperpolarized using NMR signal amplification by reversible exchange in shield enables alignment transfer to heteronuclei (SABRE-SHEATH). A 15N NMR signal enhancement of ∼2000-fold at 9.4 T is reported using parahydrogen gas (∼50% para-) and ∼0.1 M imidazole-15N2 in methanol:aqueous buffer (∼1:1). Proton binding to a 15N site of imidazole occurs at physiological pH (pKa ∼ 7.0), and the binding event changes the 15N isotropic chemical shift by ∼30 ppm. These properties are ideal for in vivo pH sensing. Additionally, imidazoles have low toxicity and are readily incorporated into a wide range of biomolecules. 15N-Imidazole SABRE-SHEATH hyperpolarization potentially enables pH sensing on scales ranging from peptide and protein molecules to living organisms.

SUBMITTER: Shchepin RV 

PROVIDER: S-EPMC4924567 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature

altmetric image

Publications

<sup>15</sup>N Hyperpolarization of Imidazole-<sup>15</sup>N<sub>2</sub> for Magnetic Resonance pH Sensing via SABRE-SHEATH.

Shchepin Roman V RV   Barskiy Danila A DA   Coffey Aaron M AM   Theis Thomas T   Shi Fan F   Warren Warren S WS   Goodson Boyd M BM   Chekmenev Eduard Y EY  

ACS sensors 20160414 6


<sup>15</sup>N nuclear spins of imidazole-<sup>15</sup>N<sub>2</sub> were hyperpolarized using NMR signal amplification by reversible exchange in shield enables alignment transfer to heteronuclei (SABRE-SHEATH). A <sup>15</sup>N NMR signal enhancement of ∼2000-fold at 9.4 T is reported using parahydrogen gas (∼50% para-) and ∼0.1 M imidazole-<sup>15</sup>N<sub>2</sub> in methanol:aqueous buffer (∼1:1). Proton binding to a <sup>15</sup>N site of imidazole occurs at physiological pH (p<i>K</i><sub  ...[more]

Similar Datasets

| S-EPMC4419867 | biostudies-literature
| S-EPMC9907724 | biostudies-literature
| S-EPMC7441520 | biostudies-literature
| S-EPMC8361944 | biostudies-literature
| S-EPMC6040279 | biostudies-literature
| S-EPMC6679352 | biostudies-literature
| S-EPMC5561492 | biostudies-literature
| S-EPMC5840861 | biostudies-literature
| S-EPMC8595556 | biostudies-literature
| S-EPMC7409511 | biostudies-literature