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In vivo super-resolution RESOLFT microscopy of Drosophila melanogaster.

ABSTRACT: Despite remarkable developments in diffraction unlimited super-resolution microscopy, in vivo nanoscopy of tissues and model organisms is still not satisfactorily established and rarely realized. RESOLFT nanoscopy is particularly suited for live cell imaging because it requires relatively low light levels to overcome the diffraction barrier. Previously, we introduced the reversibly switchable fluorescent protein rsEGFP2, which facilitated fast RESOLFT nanoscopy (Grotjohann et al., 2012). In that study, as in most other nanoscopy studies, only cultivated single cells were analyzed. Here, we report on the use of rsEGFP2 for live-cell RESOLFT nanoscopy of sub-cellular structures of intact Drosophila melanogaster larvae and of resected tissues. We generated flies expressing fusion proteins of alpha-tubulin and rsEGFP2 highlighting the microtubule cytoskeleton in all cells. By focusing through the intact larval cuticle, we achieved lateral resolution of.

SUBMITTER: Schnorrenberg S

PROVIDER: S-EPMC4927295 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature

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In vivo super-resolution RESOLFT microscopy of Drosophila melanogaster.

Schnorrenberg Sebastian S Grotjohann Tim T Vorbrüggen Gerd G Herzig Alf A Hell Stefan W SW Jakobs Stefan S

eLife 20160629

Despite remarkable developments in diffraction unlimited super-resolution microscopy, in vivo nanoscopy of tissues and model organisms is still not satisfactorily established and rarely realized. RESOLFT nanoscopy is particularly suited for live cell imaging because it requires relatively low light levels to overcome the diffraction barrier. Previously, we introduced the reversibly switchable fluorescent protein rsEGFP2, which facilitated fast RESOLFT nanoscopy (Grotjohann et al., 2012). In that ...[more]

PMID: 27355614

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