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Expansion microscopy with conventional antibodies and fluorescent proteins.

ABSTRACT: Expansion microscopy is a technique in which fluorophores on fixed specimens are linked to a swellable polymer that is physically expanded to enable super-resolution microscopy with ordinary microscopes. We have developed and characterized new methods for linking fluorophores to the polymer that now enable expansion microscopy with conventional fluorescently labeled antibodies and fluorescent proteins. Our methods simplify the procedure and expand the palette of compatible labels, allowing rapid dissemination of the technique.

SUBMITTER: Chozinski TJ

PROVIDER: S-EPMC4929147 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature

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Expansion microscopy with conventional antibodies and fluorescent proteins.

Chozinski Tyler J TJ Halpern Aaron R AR Okawa Haruhisa H Kim Hyeon-Jin HJ Tremel Grant J GJ Wong Rachel O L RO Vaughan Joshua C JC

Nature methods 20160411 6

Expansion microscopy is a technique in which fluorophores on fixed specimens are linked to a swellable polymer that is physically expanded to enable super-resolution microscopy with ordinary microscopes. We have developed and characterized new methods for linking fluorophores to the polymer that now enable expansion microscopy with conventional fluorescently labeled antibodies and fluorescent proteins. Our methods simplify the procedure and expand the palette of compatible labels, allowing rapid ...[more]

PMID: 27064647

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