Dataset Information

Antennal transcriptome and differential expression of olfactory genes in the yellow peach moth, Conogethes punctiferalis (Lepidoptera: Crambidae).

ABSTRACT: The yellow peach moth (YPM), Conogethes punctiferalis (Guenée), is a multivoltine insect pest of crops and fruits. Antennal-expressed receptors are important for insects to detect olfactory cues for host finding, mate attraction and oviposition site selection. However, few olfactory related genes were reported in YPM until now. In the present study, we sequenced and characterized the antennal transcriptomes of male and female YPM. In total, 15 putative odorant binding proteins (OBPs), 46 putative odorant receptors (ORs) and 7 putative ionotropic receptors (IRs) were annotated and identified as olfactory-related genes of C. punctiferalis. Further analysis of RT-qPCR revealed that all these olfactory genes are primarily or uniquely expressed in male and female antennae. Among which, 3 OBPs (OBP4, OBP8 and PBP2) and 4 ORs (OR22, OR26, OR44 and OR46) were specially expressed in male antennae, whereas 4 ORs (OR5, OR16, OR25 and OR42) were primarily expressed in female antennae. The predicted protein sequences were compared with homologs in other lepidopteran species and model insects, which showed high sequence homologies between C. punctiferalis and O. furnacalis. Our work allows for further functional studies of pheromone and general odorant detection genes, which might be meaningful targets for pest management.

SUBMITTER: Jia XJ

PROVIDER: S-EPMC4929561 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature

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Antennal transcriptome and differential expression of olfactory genes in the yellow peach moth, Conogethes punctiferalis (Lepidoptera: Crambidae).

Jia Xiao-Jian XJ Wang Hai-Xiang HX Yan Zeng-Guang ZG Zhang Min-Zhao MZ Wei Chun-Hua CH Qin Xiao-Chun XC Ji Wei-Rong WR Falabella Patrizia P Du Yan-Li YL

Scientific reports 20160701

The yellow peach moth (YPM), Conogethes punctiferalis (Guenée), is a multivoltine insect pest of crops and fruits. Antennal-expressed receptors are important for insects to detect olfactory cues for host finding, mate attraction and oviposition site selection. However, few olfactory related genes were reported in YPM until now. In the present study, we sequenced and characterized the antennal transcriptomes of male and female YPM. In total, 15 putative odorant binding proteins (OBPs), 46 putativ ...[more]

PMID: 27364081

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Project description:Suitability of plant tissues as food for insects varies from plant to plant. In lepidopteran insects, fitness is largely dependent on the host-finding ability of the females. Existing studies have suggested that polyphagous lepidopterans preferentially select certain host plant species for oviposition. However, the mechanisms for host recognition and selection have not been fully elucidated. For the polyphagous yellow peach moth Conogethes punctiferalis, we explored the effect of chestnut cultivar on the performance and fitness and addressed the mechanisms of plant-volatile-mediated host recognition. By carrying out laboratory experiments and field investigation on four chestnut Castanea mollissima cultivars (Huaihuang, Huaijiu, Yanhong, and Shisheng), we found that C. punctiferalis females preferentially select Huaijiu for oviposition and infestation, and caterpillars fed on Huaijiu achieved slightly greater fitness than those fed on the other three chestnut cultivars, indicating that Huaijiu was a better suitable host for C. punctiferalis. Plant volatiles played important roles in host recognition by C. punctiferalis. All seven chestnut volatile compounds, ?-pinene, camphene, ?-thujene, ?-pinene, eucalyptol, 3-carene, and nonanal, could trigger EAG responses in C. punctiferalis. The ubiquitous plant terpenoids, ?-pinene, camphene and ?-pinene, and their specific combination at concentrations and proportions similar to the emissions from the four chestnut cultivars, was sufficient to elicit host recognition behavior of female C. punctiferalis. Nonanal and a mixture containing nonanal, that mimicked the emission of C. punctiferalis infested chestnut fruits, caused avoidance response. The outcome demonstrates the effects of chestnut cultivars on the performance of C. punctiferalis and reveals the preference-performance relationship between C. punctiferalis adults and their offspring. The observed olfactory plasticity in the plant-volatile-mediated host recognition may be important for the forming of the relationship between yellow peach moth and chestnuts since it allows the polyphagous herbivores to adjust to variation in volatile emission from their host plants.

Dataset Information

Antennal transcriptome and differential expression of olfactory genes in the yellow peach moth, Conogethes punctiferalis (Lepidoptera: Crambidae).

Publications

Antennal transcriptome and differential expression of olfactory genes in the yellow peach moth, Conogethes punctiferalis (Lepidoptera: Crambidae).

Similar Datasets

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