Dataset Information

GOBP1 Plays a Key Role in Sex Pheromones and Plant Volatiles Recognition in Yellow Peach Moth, Conogethes punctiferalis (Lepidoptera: Crambidae).

ABSTRACT: Insects recognize odorous compounds using sensory neurons organized in olfactory sensilla. The process odor detection in insects requires an ensemble of proteins, including odorant binding proteins, olfactory receptors, and odor degrading enzymes; each of them are encoded by multigene families. Most functional proteins seem to be broadly tuned, responding to multiple chemical compounds with different, but mostly quite similar structures. Based on the hypothesis that insects recognize host volatiles by means of general odorant binding proteins (GOBPs), the current study aimed to characterize GOBPs of the yellow peach moth, Conogethes punctiferalis (Guenée). In oviposition preference tests, it was found that the yellow peach moth preferred volatiles from Prunus persica (peach) in finding their host plant. Exposure of the moth to volatiles from peaches affected the expression level of GOBP genes. Binding affinity of GOBPs from yellow peach moth was assessed for 16 host plant volatiles and 2 sex pheromones. The fluorescence ligand-binding assays revealed highest affinities for hexadecanal, farnesol, and limonene with KD values of 0.55 ± 0.08, 0.35 ± 0.04, and 1.54 ± 0.39, respectively. The binding sites of GOBPs from yellow peach moth were predicted using homology modeling and characterized using molecular docking approaches. The results indicated the best binding affinity of both GOBP1 and GOBP2 for farnesol, with scores of -7.4 and -8.5 kcal/mol. Thus, GOBPs may play an important role in the process of finding host plants.

SUBMITTER: Jing D

PROVIDER: S-EPMC6780721 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

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Publications

GOBP1 Plays a Key Role in Sex Pheromones and Plant Volatiles Recognition in Yellow Peach Moth, <i>Conogethes punctiferalis</i> (Lepidoptera: Crambidae).

Jing Dapeng D Zhang Tiantao T Bai Shuxiong S Prabu Sivaprasath S He Kanglai K Dewer Youssef Y Wang Zhenying Z

Insects 20190917 9

Insects recognize odorous compounds using sensory neurons organized in olfactory sensilla. The process odor detection in insects requires an ensemble of proteins, including odorant binding proteins, olfactory receptors, and odor degrading enzymes; each of them are encoded by multigene families. Most functional proteins seem to be broadly tuned, responding to multiple chemical compounds with different, but mostly quite similar structures. Based on the hypothesis that insects recognize host volati ...[more]

PMID: 31533342

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Project description:Suitability of plant tissues as food for insects varies from plant to plant. In lepidopteran insects, fitness is largely dependent on the host-finding ability of the females. Existing studies have suggested that polyphagous lepidopterans preferentially select certain host plant species for oviposition. However, the mechanisms for host recognition and selection have not been fully elucidated. For the polyphagous yellow peach moth Conogethes punctiferalis, we explored the effect of chestnut cultivar on the performance and fitness and addressed the mechanisms of plant-volatile-mediated host recognition. By carrying out laboratory experiments and field investigation on four chestnut Castanea mollissima cultivars (Huaihuang, Huaijiu, Yanhong, and Shisheng), we found that C. punctiferalis females preferentially select Huaijiu for oviposition and infestation, and caterpillars fed on Huaijiu achieved slightly greater fitness than those fed on the other three chestnut cultivars, indicating that Huaijiu was a better suitable host for C. punctiferalis. Plant volatiles played important roles in host recognition by C. punctiferalis. All seven chestnut volatile compounds, ?-pinene, camphene, ?-thujene, ?-pinene, eucalyptol, 3-carene, and nonanal, could trigger EAG responses in C. punctiferalis. The ubiquitous plant terpenoids, ?-pinene, camphene and ?-pinene, and their specific combination at concentrations and proportions similar to the emissions from the four chestnut cultivars, was sufficient to elicit host recognition behavior of female C. punctiferalis. Nonanal and a mixture containing nonanal, that mimicked the emission of C. punctiferalis infested chestnut fruits, caused avoidance response. The outcome demonstrates the effects of chestnut cultivars on the performance of C. punctiferalis and reveals the preference-performance relationship between C. punctiferalis adults and their offspring. The observed olfactory plasticity in the plant-volatile-mediated host recognition may be important for the forming of the relationship between yellow peach moth and chestnuts since it allows the polyphagous herbivores to adjust to variation in volatile emission from their host plants.

Dataset Information

GOBP1 Plays a Key Role in Sex Pheromones and Plant Volatiles Recognition in Yellow Peach Moth, Conogethes punctiferalis (Lepidoptera: Crambidae).

Publications

GOBP1 Plays a Key Role in Sex Pheromones and Plant Volatiles Recognition in Yellow Peach Moth, <i>Conogethes punctiferalis</i> (Lepidoptera: Crambidae).

Similar Datasets

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