Cell-free expression of functional receptor tyrosine kinases.
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ABSTRACT:
SUBMITTER: He W
PROVIDER: S-EPMC4929682 | biostudies-literature | 2015 Aug
REPOSITORIES: biostudies-literature
Publications
Cell-free expression of functional receptor tyrosine kinases.
He Wei W Scharadin Tiffany M TM Saldana Matthew M Gellner Candice C Hoang-Phou Steven S Takanishi Christina C Hura Gregory L GL Tainer John A JA Carraway Kermit L KL Henderson Paul T PT Coleman Matthew A MA
Scientific reports 20150814
Receptor tyrosine kinases (RTKs) play critical roles in physiological and pathological processes, and are important anticancer drug targets. In vitro mechanistic and drug discovery studies of full-length RTKs require protein that is both fully functional and free from contaminating proteins. Here we describe a rapid cell-free and detergent-free co-translation method for producing full-length and functional ERBB2 and EGFR receptor tyrosine kinases supported by water-soluble apolipoprotein A-I bas ...[more]
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