Ontology highlight
ABSTRACT:
SUBMITTER: He W
PROVIDER: S-EPMC4929682 | biostudies-literature | 2015 Aug
REPOSITORIES: biostudies-literature
Scientific reports 20150814
Receptor tyrosine kinases (RTKs) play critical roles in physiological and pathological processes, and are important anticancer drug targets. In vitro mechanistic and drug discovery studies of full-length RTKs require protein that is both fully functional and free from contaminating proteins. Here we describe a rapid cell-free and detergent-free co-translation method for producing full-length and functional ERBB2 and EGFR receptor tyrosine kinases supported by water-soluble apolipoprotein A-I bas ...[more]