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NEDDylation promotes stress granule assembly.

ABSTRACT: Stress granules (SGs) harbour translationally stalled messenger ribonucleoproteins and play important roles in regulating gene expression and cell fate. Here we show that neddylation promotes SG assembly in response to arsenite-induced oxidative stress. Inhibition or depletion of key components of the neddylation machinery concomitantly inhibits stress-induced polysome disassembly and SG assembly. Affinity purification and subsequent mass-spectrometric analysis of Nedd8-conjugated proteins from translationally stalled ribosomal fractions identified ribosomal proteins, translation factors and RNA-binding proteins (RBPs), including SRSF3, a previously known SG regulator. We show that SRSF3 is selectively neddylated at Lys85 in response to arsenite. A non-neddylatable SRSF3 (K85R) mutant do not prevent arsenite-induced polysome disassembly, but fails to support the SG assembly, suggesting that the neddylation pathway plays an important role in SG assembly.

SUBMITTER: Jayabalan AK

PROVIDER: S-EPMC4935812 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature

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NEDDylation promotes stress granule assembly.

Jayabalan Aravinth Kumar AK Sanchez Anthony A Park Ra Young RY Yoon Sang Pil SP Kang Gum-Yong GY Baek Je-Hyun JH Anderson Paul P Kee Younghoon Y Ohn Takbum T

Nature communications 20160706

Stress granules (SGs) harbour translationally stalled messenger ribonucleoproteins and play important roles in regulating gene expression and cell fate. Here we show that neddylation promotes SG assembly in response to arsenite-induced oxidative stress. Inhibition or depletion of key components of the neddylation machinery concomitantly inhibits stress-induced polysome disassembly and SG assembly. Affinity purification and subsequent mass-spectrometric analysis of Nedd8-conjugated proteins from ...[more]

PMID: 27381497

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