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Identification of methylated deoxyadenosines in vertebrates reveals diversity in DNA modifications.

ABSTRACT: Methylation of cytosine deoxynucleotides generates 5-methylcytosine (m(5)dC), a well-established epigenetic mark. However, in higher eukaryotes much less is known about modifications affecting other deoxynucleotides. Here, we report the detection of N(6)-methyldeoxyadenosine (m(6)dA) in vertebrate DNA, specifically in Xenopus laevis but also in other species including mouse and human. Our methylome analysis reveals that m(6)dA is widely distributed across the eukaryotic genome and is present in different cell types but is commonly depleted from gene exons. Thus, direct DNA modifications might be more widespread than previously thought.

SUBMITTER: Koziol MJ

PROVIDER: S-EPMC4941928 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature

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Identification of methylated deoxyadenosines in vertebrates reveals diversity in DNA modifications.

Koziol Magdalena J MJ Bradshaw Charles R CR Allen George E GE Costa Ana S H ASH Frezza Christian C Gurdon John B JB

Nature structural & molecular biology 20151221 1

Methylation of cytosine deoxynucleotides generates 5-methylcytosine (m(5)dC), a well-established epigenetic mark. However, in higher eukaryotes much less is known about modifications affecting other deoxynucleotides. Here, we report the detection of N(6)-methyldeoxyadenosine (m(6)dA) in vertebrate DNA, specifically in Xenopus laevis but also in other species including mouse and human. Our methylome analysis reveals that m(6)dA is widely distributed across the eukaryotic genome and is present in ...[more]

PMID: 26689968

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