Extracellular Recordings of Patterned Human Pluripotent Stem Cell-Derived Cardiomyocytes on Aligned Fibers.
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ABSTRACT:
SUBMITTER: Li J
PROVIDER: S-EPMC4942673 | biostudies-literature | 2016
REPOSITORIES: biostudies-literature
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Extracellular Recordings of Patterned Human Pluripotent Stem Cell-Derived Cardiomyocytes on Aligned Fibers.
Li Junjun J Minami Itsunari I Yu Leqian L Tsuji Kiyotaka K Nakajima Minako M Qiao Jing J Suzuki Masato M Shimono Ken K Nakatsuji Norio N Kotera Hitetoshi H Liu Li L Chen Yong Y
Stem cells international 20160629
Human induced pluripotent stem cell (hiPSC) derived cardiomyocytes (CMs) hold high potential for use in drug assessment and myocardial regeneration. To create tissue-like constructs of CMs for extracellular monitoring, we placed aligned fibers (AFs) on the surface of a microelectrode array and then seeded hiPSC-CMs for subsequent monitoring for 14 days. As expected, the CMs organized into anisotropic and matured tissue and the extracellular recordings showed reduced premature beating higher sign ...[more]
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