Ontology highlight
ABSTRACT:
SUBMITTER: Carlson LA
PROVIDER: S-EPMC4946900 | biostudies-literature | 2016 Jun
REPOSITORIES: biostudies-literature
Carlson Lars-Anders LA Bai Yun Y Keane Sarah C SC Doudna Jennifer A JA Hurley James H JH
eLife 20160625
HIV-1 Gag selects and packages a dimeric, unspliced viral RNA in the context of a large excess of cytosolic human RNAs. As Gag assembles on the plasma membrane, the HIV-1 genome is enriched relative to cellular RNAs by an unknown mechanism. We used a minimal system consisting of purified RNAs, recombinant HIV-1 Gag and giant unilamellar vesicles to recapitulate the selective packaging of the 5' untranslated region of the HIV-1 genome in the presence of excess competitor RNA. Mutations in the CA- ...[more]