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Dissection of specific binding of HIV-1 Gag to the 'packaging signal' in viral RNA.


ABSTRACT: Selective packaging of HIV-1 genomic RNA (gRNA) requires the presence of a cis-acting RNA element called the 'packaging signal' (?). However, the mechanism by which ? promotes selective packaging of the gRNA is not well understood. We used fluorescence correlation spectroscopy and quenching data to monitor the binding of recombinant HIV-1 Gag protein to Cy5-tagged 190-base RNAs. At physiological ionic strength, Gag binds with very similar, nanomolar affinities to both ?-containing and control RNAs. We challenged these interactions by adding excess competing tRNA; introducing mutations in Gag; or raising the ionic strength. These modifications all revealed high specificity for ?. This specificity is evidently obscured in physiological salt by non-specific, predominantly electrostatic interactions. This nonspecific activity was attenuated by mutations in the MA, CA, and NC domains, including CA mutations disrupting Gag-Gag interaction. We propose that gRNA is selectively packaged because binding to ? nucleates virion assembly with particular efficiency.

SUBMITTER: Comas-Garcia M 

PROVIDER: S-EPMC5531834 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature

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Dissection of specific binding of HIV-1 Gag to the 'packaging signal' in viral RNA.

Comas-Garcia Mauricio M   Datta Siddhartha Ak SA   Baker Laura L   Varma Rajat R   Gudla Prabhakar R PR   Rein Alan A  

eLife 20170720


Selective packaging of HIV-1 genomic RNA (gRNA) requires the presence of a <i>cis</i>-acting RNA element called the 'packaging signal' (Ψ). However, the mechanism by which Ψ promotes selective packaging of the gRNA is not well understood. We used fluorescence correlation spectroscopy and quenching data to monitor the binding of recombinant HIV-1 Gag protein to Cy5-tagged 190-base RNAs. At physiological ionic strength, Gag binds with very similar, nanomolar affinities to both Ψ-containing and con  ...[more]

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