Ontology highlight
ABSTRACT:
SUBMITTER: Faden F
PROVIDER: S-EPMC4961840 | biostudies-literature | 2016 Jul
REPOSITORIES: biostudies-literature
Faden Frederik F Ramezani Thomas T Mielke Stefan S Almudi Isabel I Nairz Knud K Froehlich Marceli S MS Höckendorff Jörg J Brandt Wolfgang W Hoehenwarter Wolfgang W Dohmen R Jürgen RJ Schnittger Arp A Dissmeyer Nico N
Nature communications 20160722
Phenotypes on-demand generated by controlling activation and accumulation of proteins of interest are invaluable tools to analyse and engineer biological processes. While temperature-sensitive alleles are frequently used as conditional mutants in microorganisms, they are usually difficult to identify in multicellular species. Here we present a versatile and transferable, genetically stable system based on a low-temperature-controlled N-terminal degradation signal (lt-degron) that allows reversib ...[more]