Ontology highlight
ABSTRACT:
SUBMITTER: York AG
PROVIDER: S-EPMC3462167 | biostudies-literature | 2012 May
REPOSITORIES: biostudies-literature
York Andrew G AG Parekh Sapun H SH Dalle Nogare Damian D Fischer Robert S RS Temprine Kelsey K Mione Marina M Chitnis Ajay B AB Combs Christian A CA Shroff Hari H
Nature methods 20120513 7
We demonstrate three-dimensional (3D) super-resolution in live multicellular organisms using structured illumination microscopy (SIM). Sparse multifocal illumination patterns generated by a digital micromirror device (DMD) allowed us to physically reject out-of-focus light, enabling 3D subdiffractive imaging in samples eightfold thicker than had been previously imaged with SIM. We imaged samples at one 2D image per second, at resolutions as low as 145 nm laterally and 400 nm axially. In addition ...[more]