Screening of ?-Tocopherol Transfer Protein Sensitive Genes in Human Hepatoma Cells (HepG2).

ABSTRACT: ?-Tocopherol transfer protein (?-TTP) is a ~32 kDa protein expressed mainly in hepatocytes. The major function of the protein is to bind specifically to ?-tocopherol and, together, the complex transfers from late lysosomes to the cell membrane. A previous study indicated that some factors might be required in the transferring process. However, there is little information available about the potential transferring factors. In addition, there remains much to learn about other physiological processes which ?-TTP might participate in. Thus, in this study a human ?-TTP eukaryotic expression vector was successfully constructed and expressed in human hepatoma cells (HepG2). The sensitive genes related to ?-TTP were then screened by microarray technology. Results showed that expression of the vector in HepG2 cells led to the identification of 323 genes showing differential expression. The differentially expressed transcripts were divided into four main categories, including (1) cell inflammation; (2) cell cycle and cell apoptosis; (3) cell signaling and gene regulation; and (4) cellular movement. A few cellular movement related transcripts were selected and verified by quantitative real-time PCR. Expressions of some were significantly increased in ?-TTP-expressed group, which indicated that these factors were likely to play a role in the transferring process.

SUBMITTER: Qu YH 

PROVIDER: S-EPMC4964392 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature