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Data on biochemical fluxes generated from biofabricated enzyme complexes assembled through engineered tags and microbial transglutaminase.

ABSTRACT: Data presented is related to an article titled "Modular construction of multi-subunit protein complexes using engineered tags and microbial transglutaminase" (Bhokisham et al., 2016) [1]. In this article, we have presented western blot and flux data associated with assembly of Pfs-LuxS enzyme complexes on beads using uni-tagged and bi-tagged LuxS enzymes. We have also presented biochemical flux following changes in enzyme stoichiometries. We covalently coupled a Pfs-LuxS complex with Protein G, an antibody binding non-enzyme component and directed these complexes to the surfaces of bacterial cells via anti-Escherichia coli antibodies. Fluorescence microscopy images represented the altered behavior of bacterial cells in response to the autoinducer-2 that is synthesized by the Protein G-enzyme complexes.

SUBMITTER: Bhokisham N

PROVIDER: S-EPMC4969242 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature

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Data on biochemical fluxes generated from biofabricated enzyme complexes assembled through engineered tags and microbial transglutaminase.

Bhokisham Narendranath N Pakhchanian Haig H Quan David D Tschirhart Tanya T Tsao Chen-Yu CY Payne Gregory F GF Bentley William E WE

Data in brief 20160709

Data presented is related to an article titled "Modular construction of multi-subunit protein complexes using engineered tags and microbial transglutaminase" (Bhokisham et al., 2016) [1]. In this article, we have presented western blot and flux data associated with assembly of Pfs-LuxS enzyme complexes on beads using uni-tagged and bi-tagged LuxS enzymes. We have also presented biochemical flux following changes in enzyme stoichiometries. We covalently coupled a Pfs-LuxS complex with Protein G, ...[more]

PMID: 27508259

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