Ontology highlight
ABSTRACT:
SUBMITTER: Boudreau C
PROVIDER: S-EPMC4971503 | biostudies-literature | 2016 Aug
REPOSITORIES: biostudies-literature
Boudreau Colton C Wee Tse-Luen Erika TL Duh Yan-Rung Silvia YR Couto Melissa P MP Ardakani Kimya H KH Brown Claire M CM
Scientific reports 20160803
It is important to determine the most effective method of delivering light onto a specimen for minimal light induced damage. Assays are presented to measure photo-bleaching of fluorophores and photo-toxicity to living cells under different illumination conditions. Turning the light off during part of the experimental time reduced photo-bleaching in a manner proportional to the time of light exposure. The rate of photo-bleaching of EGFP was reduced by 9-fold with light pulsing on the micro-second ...[more]