Ontology highlight
ABSTRACT:
SUBMITTER: Parsons TB
PROVIDER: S-EPMC4973692 | biostudies-literature | 2016 Feb
REPOSITORIES: biostudies-literature
Parsons Thomas B TB Struwe Weston B WB Gault Joseph J Yamamoto Keisuke K Taylor Thomas A TA Raj Ritu R Wals Kim K Mohammed Shabaz S Robinson Carol V CV Benesch Justin L P JL Davis Benjamin G BG
Angewandte Chemie (International ed. in English) 20160112 7
Glycosylation patterns in antibodies critically determine biological and physical properties but their precise control is a significant challenge in biology and biotechnology. We describe herein the optimization of an endoglycosidase-catalyzed glycosylation of the best-selling biotherapeutic Herceptin, an anti-HER2 antibody. Precise MS analysis of the intact four-chain Ab heteromultimer reveals nonspecific, non-enzymatic reactions (glycation), which are not detected under standard denaturing con ...[more]