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Use of 16S rRNA gene sequencing for rapid confirmatory identification of Brucella isolates.

ABSTRACT: Members of the genus Brucella are categorized as biothreat agents and pose a hazard for both humans and animals. Current identification methods rely on biochemical tests that may require up to 7 days for results. We sequenced the 16S rRNA genes of 65 Brucella strains along with 17 related strains likely to present a differential diagnostic challenge. All Brucella 16S rRNA gene sequences were determined to be identical and were clearly different from the 17 related strains, suggesting that 16S rRNA gene sequencing is a reliable tool for rapid genus-level identification of Brucella spp. and their differentiation from closely related organisms.

SUBMITTER: Gee JE

PROVIDER: S-EPMC497563 | biostudies-literature | 2004 Aug

REPOSITORIES: biostudies-literature

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Use of 16S rRNA gene sequencing for rapid confirmatory identification of Brucella isolates.

Gee Jay E JE De Barun K BK Levett Paul N PN Whitney Anne M AM Novak Ryan T RT Popovic Tanja T

Journal of clinical microbiology 20040801 8

Members of the genus Brucella are categorized as biothreat agents and pose a hazard for both humans and animals. Current identification methods rely on biochemical tests that may require up to 7 days for results. We sequenced the 16S rRNA genes of 65 Brucella strains along with 17 related strains likely to present a differential diagnostic challenge. All Brucella 16S rRNA gene sequences were determined to be identical and were clearly different from the 17 related strains, suggesting that 16S rR ...[more]

PMID: 15297511

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