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Proteomic and genomic analysis reveals novel Campylobacter jejuni outer membrane proteins and potential heterogeneity.


ABSTRACT: Gram-negative bacterial outer membrane proteins play important roles in the interaction of bacteria with their environment including nutrient acquisition, adhesion and invasion, and antibiotic resistance. In this study we identified 47 proteins within the Sarkosyl-insoluble fraction of Campylobacter jejuni 81-176, using LC-ESI-MS/MS. Comparative analysis of outer membrane protein sequences was visualised to reveal protein distribution within a panel of Campylobacter spp., identifying several C. jejuni-specific proteins. Smith-Waterman analyses of C. jejuni homologues revealed high sequence conservation amongst a number of hypothetical proteins, sequence heterogeneity of other proteins and several proteins which are absent in a proportion of strains.

SUBMITTER: Watson E 

PROVIDER: S-EPMC4975774 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature

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Proteomic and genomic analysis reveals novel <i>Campylobacter jejuni</i> outer membrane proteins and potential heterogeneity.

Watson Eleanor E   Sherry Aileen A   Inglis Neil F NF   Lainson Alex A   Jyothi Dushyanth D   Yaga Raja R   Manson Erin E   Imrie Lisa L   Everest Paul P   Smith David G E DG  

EuPA open proteomics 20140626


Gram-negative bacterial outer membrane proteins play important roles in the interaction of bacteria with their environment including nutrient acquisition, adhesion and invasion, and antibiotic resistance. In this study we identified 47 proteins within the Sarkosyl-insoluble fraction of <i>Campylobacter jejuni</i> 81-176, using LC-ESI-MS/MS. Comparative analysis of outer membrane protein sequences was visualised to reveal protein distribution within a panel of <i>Campylobacter</i> spp., identifyi  ...[more]

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