Dataset Information

Data on biochemical indexes of HFD-fed mice treatment with metformin or resveratrol.

ABSTRACT: To investigate the changes of physiological and biochemical indexes, male mice were fed a regular diet or short time high fat diet (HFD) for 10 days with oral administration of saline, metformin, resveratrol, or injected intraperitoneally (ip) with digoxin respectively every day. Food intake and body weight were recorded simultaneously. Blood was collected after mice were sacrificed and then tested with commercial kits. The data manifested that metformin and resveratrol only ameliorate free fatty acids and glycerol in HFD-fed mice. Data interpretation of this part can be found in the research article "Metformin and resveratrol ameliorate muscle insulin resistance through preventing lipolysis and inflammation in hypoxic adipose tissue" (Zhao et al.,) [1].

SUBMITTER: Zhao W

PROVIDER: S-EPMC4979042 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Data on biochemical indexes of HFD-fed mice treatment with metformin or resveratrol.

Zhao Wenjun W Li Aiyun A Feng Xin X Hou Ting T Liu Kang K Liu Baolin B Zhang Ning N

Data in brief 20160729

To investigate the changes of physiological and biochemical indexes, male mice were fed a regular diet or short time high fat diet (HFD) for 10 days with oral administration of saline, metformin, resveratrol, or injected intraperitoneally (ip) with digoxin respectively every day. Food intake and body weight were recorded simultaneously. Blood was collected after mice were sacrificed and then tested with commercial kits. The data manifested that metformin and resveratrol only ameliorate free fatt ...[more]

PMID: 27547796

Similar Datasets

Project description:The data presented here come from the article "Histomorphometric evaluation of the rat kidney submitted to warm ischemia and the protective effect of resveratrol" [1]. Rats of Wistar lineage (n?=?39; 9 weeks of age) were obtained and apportioned into 4 groups at random. Both groups Sham (S) and Sham Resveratrol (SR) were submitted to open laparotomy and dissection of the left renal pedicle, the same as groups Ischemia (I) and Ischemia Resveratrol (IR), being the last two also submitted to 1 h left warm renal ischemia. SR and IR were treated with 30?mg/kg of resveratrol intraperitoneally 1 h before the surgical procedure, while S and I received saline injections. Rats were killed a month after surgery by anesthetic overdose. A blood sample was collected by cardiac puncture for determination of serum urea and creatinine serum by biochemical analysis at automated enzymatic method. Kidneys were weighted, Sherle´s method was used for measurement of their volume and then both were fixated in buffered formalin for 48 h. Cortex-non-cortex areas ratio (C-NC) was assessed by Cavalieri's method using a stereoscope. The product of multiplying the renal volume by the C-NC is the cortical volume (CV). Left kidneys fragments were processed for histology resulting in slides that were stained with haematoxylin and eosin. For histomorphometric analyses, 25 random cortical fields were photographed at 200x magnification using a camera attached to a light microscope. The estimation of glomerular volumetric density (Vv [Glom]), indication of proportional volume occupied by glomeruli in the cortex, was performed by the point-counting method. The point-sampled intercepts method was used to estimate the volume-weighted mean glomerular volume (VWGV). Total number of glomeruli per kidney (N [Glom]) estimation was achieved through the formula CVxVv [Glom]/VWGV. All the data were tabulated in spreadsheets. The quantitative results were compared by one-way ANOVA with Tukey's post-test using GraphPad Prism software. All results were considered significant when the value of p <0.05.

Dataset Information

Data on biochemical indexes of HFD-fed mice treatment with metformin or resveratrol.

Publications

Data on biochemical indexes of HFD-fed mice treatment with metformin or resveratrol.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets