Ontology highlight
ABSTRACT:
SUBMITTER: Ye J
PROVIDER: S-EPMC4984262 | biostudies-literature | 2014 Jul
REPOSITORIES: biostudies-literature
Ye Jun J Nadar S Venkadesh SV Li Jiaojiao J Rosen Barry P BP
Acta crystallographica. Section D, Biological crystallography 20140629 Pt 7
The structure of glutaredoxin 2 (Grx2) from Escherichia coli co-crystallized with glutathione (GSH) was solved at 1.60 Å resolution. The structure of a mutant with the active-site residues Cys9 and Cys12 changed to serine crystallized in the absence of glutathione was solved to 2.4 Å resolution. Grx2 has an N-terminal domain characteristic of glutaredoxins, and the overall structure is congruent with the structure of glutathione S-transferases (GSTs). Purified Grx2 exhibited GST activity. Grx2, ...[more]