MRPrimerW: a tool for rapid design of valid high-quality primers for multiple target qPCR experiments.

ABSTRACT: Design of high-quality primers for multiple target sequences is essential for qPCR experiments, but is challenging due to the need to consider both homology tests on off-target sequences and the same stringent filtering constraints on the primers. Existing web servers for primer design have major drawbacks, including requiring the use of BLAST-like tools for homology tests, lack of support for ranking of primers, TaqMan probes and simultaneous design of primers against multiple targets. Due to the large-scale computational overhead, the few web servers supporting homology tests use heuristic approaches or perform homology tests within a limited scope. Here, we describe the MRPrimerW, which performs complete homology testing, supports batch design of primers for multi-target qPCR experiment

SUBMITTER: Kim H 

PROVIDER: S-EPMC4987926 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature