Ontology highlight
ABSTRACT:
SUBMITTER: Santos DP
PROVIDER: S-EPMC4988528 | biostudies-literature | 2016 Aug
REPOSITORIES: biostudies-literature
Santos David P DP Kiskinis Evangelos E Eggan Kevin K Merkle Florian T FT
Current protocols in stem cell biology 20160817
Genome editing of human pluripotent stem cells (hPSCs) with the CRISPR/Cas9 system has the potential to revolutionize hPSC-based disease modeling, drug screening, and transplantation therapy. Here, we aim to provide a single resource to enable groups, even those with limited experience with hPSC culture or the CRISPR/Cas9 system, to successfully perform genome editing. The methods are presented in detail and are supported by a theoretical framework to allow for the incorporation of inevitable im ...[more]