Ontology highlight
ABSTRACT:
SUBMITTER: Gupta N
PROVIDER: S-EPMC6060633 | biostudies-literature | 2018 May
REPOSITORIES: biostudies-literature
Gupta Navin N Susa Koichiro K Yoda Yoko Y Bonventre Joseph V JV Valerius M Todd MT Morizane Ryuji R
Current protocols in stem cell biology 20180426 1
Human pluripotent stem cells (hPSCs) represent a formidable tool for disease modeling, drug discovery, and regenerative medicine using human cells and tissues in vitro. Evolving techniques of targeted genome editing, specifically the CRISPR/Cas9 system, allow for the generation of cell lines bearing gene-specific knock-outs, knock-in reporters, and precise mutations. However, there are increasing concerns related to the transfection efficiency, cell viability, and maintenance of pluripotency pro ...[more]