Ontology highlight
ABSTRACT:
SUBMITTER: Singharoy A
PROVIDER: S-EPMC4990421 | biostudies-literature | 2016 Jul
REPOSITORIES: biostudies-literature
Singharoy Abhishek A Teo Ivan I McGreevy Ryan R Stone John E JE Zhao Jianhua J Schulten Klaus K
eLife 20160707
Two structure determination methods, based on the molecular dynamics flexible fitting (MDFF) paradigm, are presented that resolve sub-5 Å cryo-electron microscopy (EM) maps with either single structures or ensembles of such structures. The methods, denoted cascade MDFF and resolution exchange MDFF, sequentially re-refine a search model against a series of maps of progressively higher resolutions, which ends with the original experimental resolution. Application of sequential re-refinement enable ...[more]