Ontology highlight
ABSTRACT:
SUBMITTER: Chen Y
PROVIDER: S-EPMC4990487 | biostudies-literature | 2016 Sep
REPOSITORIES: biostudies-literature
Chen Yu Y Yang Fan F Zubovic Lorena L Pavelitz Tom T Yang Wen W Godin Katherine K Walker Matthew M Zheng Suxin S Macchi Paolo P Varani Gabriele G
Nature chemical biology 20160718 9
The RNA recognition motif (RRM) is the largest family of eukaryotic RNA-binding proteins. Engineered RRMs with well-defined specificity would provide valuable tools and an exacting test of the current understanding of specificity. We have redesigned the specificity of an RRM using rational methods and demonstrated retargeting of its activity in cells. We engineered the conserved RRM of human Rbfox proteins to specifically bind to the terminal loop of a microRNA precursor (pre-miR-21) with high a ...[more]