Unknown

Dataset Information

0

Lights, camera, action! Capturing the spliceosome and pre-mRNA splicing with single-molecule fluorescence microscopy.

ABSTRACT: The process of removing intronic sequences from a precursor to messenger RNA (pre-mRNA) to yield a mature mRNA transcript via splicing is an integral step in eukaryotic gene expression. Splicing is carried out by a cellular nanomachine called the spliceosome that is composed of RNA components and dozens of proteins. Despite decades of study, many fundamentals of spliceosome function have remained elusive. Recent developments in single-molecule fluorescence microscopy have afforded new tools to better probe the spliceosome and the complex, dynamic process of splicing by direct observation of single molecules. These cutting-edge technologies enable investigators to monitor the dynamics of specific splicing components, whole spliceosomes, and even cotranscriptional splicing within living cells. WIREs RNA 2016, 7:683-701. doi: 10.1002/wrna.1358 For further resources related to this article, please visit the WIREs website.

SUBMITTER: DeHaven AC 

PROVIDER: S-EPMC4990488 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Lights, camera, action! Capturing the spliceosome and pre-mRNA splicing with single-molecule fluorescence microscopy.

DeHaven Alexander C AC   Norden Ian S IS   Hoskins Aaron A AA  

Wiley interdisciplinary reviews. RNA 20160520 5

The process of removing intronic sequences from a precursor to messenger RNA (pre-mRNA) to yield a mature mRNA transcript via splicing is an integral step in eukaryotic gene expression. Splicing is carried out by a cellular nanomachine called the spliceosome that is composed of RNA components and dozens of proteins. Despite decades of study, many fundamentals of spliceosome function have remained elusive. Recent developments in single-molecule fluorescence microscopy have afforded new tools to b  ...[more]

Similar Datasets

Unknown Alternative spliceosome assembly pathways revealed by single-molecule fluorescence microscopy.
| S-EPMC3927372 | biostudies-literature
Unknown Identification of transient intermediates during spliceosome activation by single molecule fluorescence microscopy.
| S-EPMC9860250 | biostudies-literature
Unknown Cis-splicing and translation of the pre-trans-splicing molecule combine with efficiency in spliceosome-mediated RNA trans-splicing.
| S-EPMC4048894 | biostudies-literature
Unknown Lights! Camera! Action Projects! Engaging Psychopharmacology Students in Service-based Action Projects Focusing on Student Alcohol Abuse.
| S-EPMC4917345 | biostudies-literature
Unknown Simulations of camera-based single-molecule fluorescence experiments.
| S-EPMC5898730 | biostudies-literature
Unknown Spliceosome-Mediated Pre-mRNA trans-Splicing Can Repair CEP290 mRNA.
| S-EPMC6023944 | biostudies-literature
Unknown The spliceosome factor SART1 exerts its anti-HCV action through mRNA splicing.
| S-EPMC4404186 | biostudies-literature
Unknown Nanometer-localized multiple single-molecule fluorescence microscopy.
| S-EPMC509198 | biostudies-literature
Unknown Molecular Mechanisms of pre-mRNA Splicing through Structural Biology of the Spliceosome.
| S-EPMC6314064 | biostudies-literature
Unknown Blocking late stages of splicing quickly limits pre-spliceosome assembly in vivo.
| S-EPMC6844569 | biostudies-literature