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Combined fiber probe for fluorescence lifetime and Raman spectroscopy.

ABSTRACT: In this contribution we present a dual modality fiber optic probe combining fluorescence lifetime imaging (FLIm) and Raman spectroscopy for in vivo endoscopic applications. The presented multi-spectroscopy probe enables efficient excitation and collection of fluorescence lifetime signals for FLIm in the UV/visible wavelength region, as well as of Raman spectra in the near-IR for simultaneous Raman/FLIm imaging. The probe was characterized in terms of its lateral resolution and distance dependency of the Raman and FLIm signals. In addition, the feasibility of the probe for in vivo FLIm and Raman spectral characterization of tissue was demonstrated. Graphical Abstract An image comparison between FLIm and Raman spectroscopy acquired with the bimodal probe onseveral tissue samples.

SUBMITTER: Dochow S

PROVIDER: S-EPMC4995092 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature

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Combined fiber probe for fluorescence lifetime and Raman spectroscopy.

Dochow Sebastian S Ma Dinglong D Latka Ines I Bocklitz Thomas T Hartl Brad B Bec Julien J Fatakdawala Hussain H Marple Eric E Urmey Kirk K Wachsmann-Hogiu Sebastian S Schmitt Michael M Marcu Laura L Popp Jürgen J

Analytical and bioanalytical chemistry 20150621 27

In this contribution we present a dual modality fiber optic probe combining fluorescence lifetime imaging (FLIm) and Raman spectroscopy for in vivo endoscopic applications. The presented multi-spectroscopy probe enables efficient excitation and collection of fluorescence lifetime signals for FLIm in the UV/visible wavelength region, as well as of Raman spectra in the near-IR for simultaneous Raman/FLIm imaging. The probe was characterized in terms of its lateral resolution and distance dependenc ...[more]

PMID: 26093843

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