Ontology highlight
ABSTRACT:
SUBMITTER: James DI
PROVIDER: S-EPMC4995692 | biostudies-literature | 2016
REPOSITORIES: biostudies-literature
F1000Research 20160425
After a DNA damage signal multiple polymers of ADP ribose attached to poly(ADP) ribose (PAR) polymerases (PARPs) are broken down by the enzyme poly(ADP) ribose glycohydrolase (PARG). Inhibition of PARG leads to a failure of DNA repair and small molecule inhibition of PARG has been a goal for many years. To determine whether biochemical inhibitors of PARG are active in cells we have designed an immunofluorescence assay to detect nuclear PAR after DNA damage. This 384-well assay is suitable for me ...[more]