Dataset Information

New polymer of lactic-co-glycolic acid-modified polyethylenimine for nucleic acid delivery.

ABSTRACT:

Aim

To develop an improved delivery system for nucleic acids.

Materials & methods

We designed, synthesized and characterized a new polymer of lactic-co-glycolic acid-modified polyethylenimine (LGA-PEI). Functions of LGA-PEI polymer were determined.

Results

The new LGA-PEI polymer spontaneously formed nanoparticles (NPs) with DNA or RNA, and showed higher DNA or RNA loading efficiency, higher or comparable transfection efficacy, and lower cytotoxicity in several cell types including PANC-1, Jurkat and HEK293 cells, when compared with lipofectamine 2000, branched or linear PEI (25 kDa). In nude mouse models, LGA-PEI showed higher delivery efficiency of plasmid DNA or miRNA mimic into pancreatic and ovarian xenograft tumors. LGA-PEI/DNA NPs showed much lower toxicity than control PEI NPs in mouse models.

Conclusion

The new LGA-PEI polymer is a safer and more effective system to deliver DNA or RNA than PEI.

SUBMITTER: Lu JM

PROVIDER: S-EPMC4996154 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature

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Publications

New polymer of lactic-co-glycolic acid-modified polyethylenimine for nucleic acid delivery.

Lü Jian-Ming JM Liang Zhengdong Z Wang Xiaoxiao X Gu Jianhua J Yao Qizhi Q Chen Changyi C

Nanomedicine (London, England) 20160726 15

<h4>Aim</h4>To develop an improved delivery system for nucleic acids.<h4>Materials & methods</h4>We designed, synthesized and characterized a new polymer of lactic-co-glycolic acid-modified polyethylenimine (LGA-PEI). Functions of LGA-PEI polymer were determined.<h4>Results</h4>The new LGA-PEI polymer spontaneously formed nanoparticles (NPs) with DNA or RNA, and showed higher DNA or RNA loading efficiency, higher or comparable transfection efficacy, and lower cytotoxicity in several cell types i ...[more]

PMID: 27456396

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Project description:In the pursuit of continuous improvement in the area of biomaterial design, blends of mixed-substituent polyphosphazenes and poly (lactic acid-glycolic acid) (PLGA) were prepared, and their morphology of phase distributions for the first time was studied. The degradation mechanism and osteocompatibility of the blends were also evaluated for their use as regenerative materials. Poly [(ethyl phenylalanato)25(glycine ethyl glycinato)75phosphazene](PNEPAGEG) and poly [(glycine ethyl glycinato)75(phenylphenoxy)25phosphazene](PNGEGPhPh) were blended with PLGA at various weight ratios to yield different blends, namely PNEPAGEG-PLGA 25:75, PNEPAGEG-PLGA 50:50, PNGEGPhPh-PLGA 25:75, and PNGEGPhPh-PLGA 50:50. The molecular interactions, domain sizes, and phase distributions of the blends were confirmed by atomic force microscopy (AFM) as the PNEPAGEG-PLGA and PNGEGPhPh-PLGA blends showed different domain sizes and phase distributions. Due to the extensive hydrogen bonding within the two polymer components, PNEPAGEG-PLGA exhibited small-sized domains and well-distributed morphology. While for the PNGEGPhPh-PLGA blends, the presence of phenylphenol (PhPh) caused the formation of PLGA large-sized domains as the PLGA formed a continuous phase and PNGEGPhPh constituted a dispersed phase. In addition to AFM results, scanning electron microscopy-energy dispersive X-ray spectrometry (SEM-EDS), differential scanning calorimetry (DSC), dynamic mechanical analysis (DMA), and Fourier transform infrared spectroscopy (FTIR) results demonstrated the miscibility of the blends. The PNEPAGEG-PLGA and PNGEGPhPh-PLGA blends presented in situ 3D interconnected porous structures upon degradation in phosphate-buffered saline (PBS) media at 37°C. However, the blends showed different mechanistic pathways to the formations of the pores. The difference in the erosion patterns could be attributed to the nature of molecular attractions that exist in the blends. Furthermore, the novel blends were able to support cell growth as compared to PLGA, and accommodate cell infiltrations, which ultimately augmented surface area for better cell-material interactions.

Dataset Information

New polymer of lactic-co-glycolic acid-modified polyethylenimine for nucleic acid delivery.

Aim

Materials & methods

Results

Conclusion

Publications

New polymer of lactic-co-glycolic acid-modified polyethylenimine for nucleic acid delivery.

Similar Datasets

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