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Imaging Renal Urea Handling in Rats at Millimeter Resolution using Hyperpolarized Magnetic Resonance Relaxometry.

ABSTRACT: In vivo spin spin relaxation time (T₂) heterogeneity of hyperpolarized [¹³C,¹⁵N₂]urea in the rat kidney was investigated. Selective quenching of the vascular hyperpolarized ¹³C signal with a macromolecular relaxation agent revealed that a long-T₂ component of the [¹³C,¹⁵N₂]urea signal originated from the renal extravascular space, thus allowing the vascular and renal filtrate contrast agent pools of the [¹³C,¹⁵N₂]urea to be distinguished via multi-exponential analysis. The T₂ response to induced diuresis and antidiuresis was performed with two imaging agents: hyperpolarized [¹³C,¹⁵N₂]urea and a control agent hyperpolarized bis-1,1-(hydroxymethyl)-1-¹³C-cyclopropane-²H₈. Large T₂ increases in the inner-medullar and papilla were observed with the former agent and not the latter during antidiuresis. Therefore, [¹³C,¹⁵N₂]urea relaxometry is sensitive to two steps of the renal urea handling process: glomerular filtration and the inner-medullary urea transporter (UT)-A1 and UT-A3 mediated urea concentrating process. Simple motion correction and subspace denoising algorithms are presented to aid in the multi exponential data analysis. Furthermore, a T₂-edited, ultra long echo time sequence was developed for sub-2 mm³ resolution 3D encoding of urea by exploiting relaxation differences in the vascular and filtrate pools.

SUBMITTER: Reed GD

PROVIDER: S-EPMC4996281 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature

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Imaging Renal Urea Handling in Rats at Millimeter Resolution using Hyperpolarized Magnetic Resonance Relaxometry.

Reed Galen D GD von Morze Cornelius C Verkman Alan S AS Koelsch Bertram L BL Chaumeil Myriam M MM Lustig Michael M Ronen Sabrina M SM Bok Robert A RA Sands Jeff M JM Larson Peder E Z PE Wang Zhen J ZJ Larsen Jan Henrik Ardenkjær JH Kurhanewicz John J Vigneron Daniel B DB

Tomography (Ann Arbor, Mich.) 20160601 2

In vivo spin spin relaxation time (T2) heterogeneity of hyperpolarized [13C,15N2]urea in the rat kidney was investigated. Selective quenching of the vascular hyperpolarized 13C signal with a macromolecular relaxation agent revealed that a long-T2 component of the [13C,15N2]urea signal originated from the renal extravascular space, thus allowing the vascular and renal filtrate c ...[more]

PMID: 27570835

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Project description:BackgroundTo enable free-breathing whole-heart sub-millimeter resolution coronary magnetic resonance angiography (CMRA) in a clinically feasible scan time by combining low-rank patch-based undersampled reconstruction (3D-PROST) with a highly accelerated non-rigid motion correction framework.MethodsNon-rigid motion corrected CMRA combined with 2D image-based navigators has been previously proposed to enable 100% respiratory scan efficiency in modestly undersampled acquisitions. Achieving sub-millimeter isotropic resolution with such techniques still requires prohibitively long acquisition times. We propose to combine 3D-PROST reconstruction with a highly accelerated non-rigid motion correction framework to achieve sub-millimeter resolution CMRA in less than 10 min. Ten healthy subjects and eight patients with suspected coronary artery disease underwent 4-5-fold accelerated free-breathing whole-heart CMRA with 0.9 mm3 isotropic resolution. Vessel sharpness, vessel length and image quality obtained with the proposed non-rigid (NR) PROST approach were compared against translational correction only (TC-PROST) and a previously proposed NR motion-compensated technique (non-rigid SENSE) in healthy subjects. For the patient study, image quality scoring and visual comparison with coronary computed tomography angiography (CCTA) were performed.ResultsAverage scan times [min:s] were 6:01 ± 0:59 (healthy subjects) and 8:29 ± 1:41 (patients). In healthy subjects, vessel sharpness of the left anterior descending (LAD) and right (RCA) coronary arteries were improved with the proposed non-rigid PROST (LAD: 51.2 ± 8.8%, RCA: 61.2 ± 9.1%) in comparison to TC-PROST (LAD: 43.8 ± 5.1%, P = 0.051, RCA: 54.3 ± 8.3%, P = 0.218) and non-rigid SENSE (LAD: 46.1 ± 5.8%, P = 0.223, RCA: 56.7 ± 9.6%, P = 0.50), although differences were not statistically significant. The average visual image quality score was significantly higher for NR-PROST (LAD: 3.2 ± 0.6, RCA: 3.3 ± 0.7) compared with TC-PROST (LAD: 2.1 ± 0.6, P = 0.018, RCA: 2.0 ± 0.7, P = 0.014) and non-rigid SENSE (LAD: 2.3 ± 0.5, P = 0.008, RCA: 2.5 ± 0.7, P = 0.016). In patients, the proposed approach showed good delineation of the coronaries, in agreement with CCTA, with image quality scores and vessel sharpness similar to that of healthy subjects.ConclusionsWe demonstrate the feasibility of combining high undersampling factors with non-rigid motion-compensated reconstruction to obtain high-quality sub-millimeter isotropic CMRA images in ~ 8 min. Validation in a larger cohort of patients with coronary artery disease is now warranted.

Dataset Information

Imaging Renal Urea Handling in Rats at Millimeter Resolution using Hyperpolarized Magnetic Resonance Relaxometry.

Publications

Imaging Renal Urea Handling in Rats at Millimeter Resolution using Hyperpolarized Magnetic Resonance Relaxometry.

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