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Redox Nanodomains Are Induced by and Control Calcium Signaling at the ER-Mitochondrial Interface.

ABSTRACT: The ER-mitochondrial interface is central to calcium signaling, organellar dynamics, and lipid biosynthesis. The ER and mitochondrial membranes also host sources and targets of reactive oxygen species (ROS), but their local dynamics and relevance remained elusive since measurement and perturbation of ROS at the organellar interface has proven difficult. Employing drug-inducible synthetic ER-mitochondrial linkers, we overcame this problem and demonstrate that the ER-mitochondrial interface hosts a nanodomain of H2O2, which is induced by cytoplasmic [Ca(2+)] spikes and exerts a positive feedback on calcium oscillations. H2O2 nanodomains originate from the mitochondrial cristae, which are compressed upon calcium signal propagation to the mitochondria, likely due to Ca(2+)-induced K(+) and concomitant water influx to the matrix. Thus, ER-mitochondrial H2O2 nanodomains represent a component of inter-organelle communication, regulating calcium signaling and mitochondrial activities.

SUBMITTER: Booth DM

PROVIDER: S-EPMC4998968 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature

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Redox Nanodomains Are Induced by and Control Calcium Signaling at the ER-Mitochondrial Interface.

Booth David M DM Enyedi Balázs B Geiszt Miklós M Várnai Péter P Hajnóczky György G

Molecular cell 20160707 2

The ER-mitochondrial interface is central to calcium signaling, organellar dynamics, and lipid biosynthesis. The ER and mitochondrial membranes also host sources and targets of reactive oxygen species (ROS), but their local dynamics and relevance remained elusive since measurement and perturbation of ROS at the organellar interface has proven difficult. Employing drug-inducible synthetic ER-mitochondrial linkers, we overcame this problem and demonstrate that the ER-mitochondrial interface hosts ...[more]

PMID: 27397688

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Dataset Information

Redox Nanodomains Are Induced by and Control Calcium Signaling at the ER-Mitochondrial Interface.

Publications

Redox Nanodomains Are Induced by and Control Calcium Signaling at the ER-Mitochondrial Interface.

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