Ontology highlight
ABSTRACT:
SUBMITTER: Rodriguez EA
PROVIDER: S-EPMC5007177 | biostudies-literature | 2016 Sep
REPOSITORIES: biostudies-literature
Rodriguez Erik A EA Tran Geraldine N GN Gross Larry A LA Crisp Jessica L JL Shu Xiaokun X Lin John Y JY Tsien Roger Y RY
Nature methods 20160801 9
Far-red fluorescent proteins (FPs) are desirable for in vivo imaging because with these molecules less light is scattered, absorbed, or re-emitted by endogenous biomolecules compared with cyan, green, yellow, and orange FPs. We developed a new class of FP from an allophycocyanin α-subunit (APCα). Native APC requires a lyase to incorporate phycocyanobilin. The evolved FP, which we named small ultra-red FP (smURFP), covalently attaches a biliverdin (BV) chromophore without a lyase, and has 642/670 ...[more]