Ontology highlight
ABSTRACT:
SUBMITTER: Behnke J
PROVIDER: S-EPMC5010498 | biostudies-literature | 2016 Sep
REPOSITORIES: biostudies-literature
Behnke Julia J Mann Melissa J MJ Scruggs Fei-Lin FL Feige Matthias J MJ Hendershot Linda M LM
Molecular cell 20160818 5
Protein maturation in the endoplasmic reticulum is controlled by multiple chaperones, but how they recognize and determine the fate of their clients remains unclear. We developed an in vivo peptide library covering substrates of the ER Hsp70 system: BiP, Grp170, and three of BiP's DnaJ-family co-factors (ERdj3, ERdj4, and ERdj5). In vivo binding studies revealed that sites for pro-folding chaperones BiP and ERdj3 were frequent and dispersed throughout the clients, whereas Grp170, ERdj4, and ERdj ...[more]