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A quantitative method to measure biofilm removal efficiency from complex biomaterial surfaces using SEM and image analysis.


ABSTRACT: Biofilm accumulation on biomaterial surfaces is a major health concern and significant research efforts are directed towards producing biofilm resistant surfaces and developing biofilm removal techniques. To accurately evaluate biofilm growth and disruption on surfaces, accurate methods which give quantitative information on biofilm area are needed, as current methods are indirect and inaccurate. We demonstrate the use of machine learning algorithms to segment biofilm from scanning electron microscopy images. A case study showing disruption of biofilm from rough dental implant surfaces using cavitation bubbles from an ultrasonic scaler is used to validate the imaging and analysis protocol developed. Streptococcus mutans biofilm was disrupted from sandblasted, acid etched (SLA) Ti discs and polished Ti discs. Significant biofilm removal occurred due to cavitation from ultrasonic scaling (p?

SUBMITTER: Vyas N 

PROVIDER: S-EPMC5013386 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature

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A quantitative method to measure biofilm removal efficiency from complex biomaterial surfaces using SEM and image analysis.

Vyas N N   Sammons R L RL   Addison O O   Dehghani H H   Walmsley A D AD  

Scientific reports 20160907


Biofilm accumulation on biomaterial surfaces is a major health concern and significant research efforts are directed towards producing biofilm resistant surfaces and developing biofilm removal techniques. To accurately evaluate biofilm growth and disruption on surfaces, accurate methods which give quantitative information on biofilm area are needed, as current methods are indirect and inaccurate. We demonstrate the use of machine learning algorithms to segment biofilm from scanning electron micr  ...[more]

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