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Characterization of molecular interactions between Escherichia coli RNA polymerase and topoisomerase I by molecular simulations.

ABSTRACT: Escherichia coli topoisomerase I (EctopoI), a type IA DNA topoisomerase, relaxes the negative DNA supercoiling generated by RNA polymerase (RNAP) during transcription elongation. Due to the lack of structural information on the complex, the exact nature of the RNAP-EctopoI interactions remains unresolved. Herein, we report for the first time, the structure-based modeling of the RNAP-EctopoI interactions using computational methods. Our results predict that the salt bridge as well as hydrogen bond interactions are responsible for the formation and stabilization of the RNAP-EctopoI complex. Our investigations provide molecular insights for understanding how EctopoI interacts with RNAP, a critical step for preventing hypernegative DNA supercoiling during transcription.

SUBMITTER: Tiwari PB

PROVIDER: S-EPMC5014613 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature

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Characterization of molecular interactions between Escherichia coli RNA polymerase and topoisomerase I by molecular simulations.

Tiwari Purushottam B PB Chapagain Prem P PP Banda Srikanth S Darici Yesim Y Üren Aykut A Tse-Dinh Yuk-Ching YC

FEBS letters 20160804 17

Escherichia coli topoisomerase I (EctopoI), a type IA DNA topoisomerase, relaxes the negative DNA supercoiling generated by RNA polymerase (RNAP) during transcription elongation. Due to the lack of structural information on the complex, the exact nature of the RNAP-EctopoI interactions remains unresolved. Herein, we report for the first time, the structure-based modeling of the RNAP-EctopoI interactions using computational methods. Our results predict that the salt bridge as well as hydrogen bon ...[more]

PMID: 27448274

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