Dataset Information

MRNIP/C5orf45 Interacts with the MRN Complex and Contributes to the DNA Damage Response.

ABSTRACT: Through an RNAi-based screen for previously uncharacterized regulators of genome stability, we have identified the human protein C5orf45 as an important factor in preventing the accumulation of DNA damage in human cells. Here, we functionally characterize C5orf45 as a binding partner of the MRE11-RAD50-NBS1 (MRN) damage-sensing complex. Hence, we rename C5orf45 as MRNIP for MRN-interacting protein (MRNIP). We find that MRNIP is rapidly recruited to sites of DNA damage. Cells depleted of MRNIP display impaired chromatin loading of the MRN complex, resulting in reduced DNA end resection and defective ATM-mediated DNA damage signaling, a reduced ability to repair DNA breaks, and radiation sensitivity. Finally, we show that MRNIP phosphorylation on serine 115 leads to its nuclear localization, and this modification is required for MRNIP's role in promoting genome stability. Collectively, these data reveal that MRNIP is an important component of the human DNA damage response.

SUBMITTER: Staples CJ

PROVIDER: S-EPMC5014761 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature

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MRNIP/C5orf45 Interacts with the MRN Complex and Contributes to the DNA Damage Response.

Staples Christopher J CJ Barone Giancarlo G Myers Katie N KN Ganesh Anil A Gibbs-Seymour Ian I Patil Abhijit A AA Beveridge Ryan D RD Daye Caroline C Beniston Richard R Maslen Sarah S Ahel Ivan I Skehel J Mark JM Collis Spencer J SJ

Cell reports 20160825 10

Through an RNAi-based screen for previously uncharacterized regulators of genome stability, we have identified the human protein C5orf45 as an important factor in preventing the accumulation of DNA damage in human cells. Here, we functionally characterize C5orf45 as a binding partner of the MRE11-RAD50-NBS1 (MRN) damage-sensing complex. Hence, we rename C5orf45 as MRNIP for MRN-interacting protein (MRNIP). We find that MRNIP is rapidly recruited to sites of DNA damage. Cells depleted of MRNIP di ...[more]

PMID: 27568553

Dataset Information

MRNIP/C5orf45 Interacts with the MRN Complex and Contributes to the DNA Damage Response.

Publications

MRNIP/C5orf45 Interacts with the MRN Complex and Contributes to the DNA Damage Response.

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