SUMOFLUX: A Generalized Method for Targeted 13C Metabolic Flux Ratio Analysis.
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ABSTRACT:
SUBMITTER: Kogadeeva M
PROVIDER: S-EPMC5023139 | biostudies-literature | 2016 Sep
REPOSITORIES: biostudies-literature
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SUMOFLUX: A Generalized Method for Targeted 13C Metabolic Flux Ratio Analysis.
Kogadeeva Maria M Zamboni Nicola N
PLoS computational biology 20160914 9
Metabolic fluxes are a cornerstone of cellular physiology that emerge from a complex interplay of enzymes, carriers, and nutrients. The experimental assessment of in vivo intracellular fluxes using stable isotopic tracers is essential if we are to understand metabolic function and regulation. Flux estimation based on 13C or 2H labeling relies on complex simulation and iterative fitting; processes that necessitate a level of expertise that ordinarily preclude the non-expert user. To overcome this ...[more]
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