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VE-cadherin interacts with cell polarity protein Pals1 to regulate vascular lumen formation.


ABSTRACT: Blood vessel tubulogenesis requires the formation of stable cell-to-cell contacts and the establishment of apicobasal polarity of vascular endothelial cells. Cell polarity is regulated by highly conserved cell polarity protein complexes such as the Par3-aPKC-Par6 complex and the CRB3-Pals1-PATJ complex, which are expressed by many different cell types and regulate various aspects of cell polarity. Here we describe a functional interaction of VE-cadherin with the cell polarity protein Pals1. Pals1 directly interacts with VE-cadherin through a membrane-proximal motif in the cytoplasmic domain of VE-cadherin. VE-cadherin clusters Pals1 at cell-cell junctions. Mutating the Pals1-binding motif in VE-cadherin abrogates the ability of VE-cadherin to regulate apicobasal polarity and vascular lumen formation. In a similar way, deletion of the Par3-binding motif at the C-terminus of VE-cadherin impairs apicobasal polarity and vascular lumen formation. Our findings indicate that the biological activity of VE-cadherin in regulating endothelial polarity and vascular lumen formation is mediated through its interaction with the two cell polarity proteins Pals1 and Par3.

SUBMITTER: Brinkmann BF 

PROVIDER: S-EPMC5025268 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature

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VE-cadherin interacts with cell polarity protein Pals1 to regulate vascular lumen formation.

Brinkmann Benjamin F BF   Steinbacher Tim T   Hartmann Christian C   Kummer Daniel D   Pajonczyk Denise D   Mirzapourshafiyi Fatemeh F   Nakayama Masanori M   Weide Thomas T   Gerke Volker V   Ebnet Klaus K  

Molecular biology of the cell 20160727 18


Blood vessel tubulogenesis requires the formation of stable cell-to-cell contacts and the establishment of apicobasal polarity of vascular endothelial cells. Cell polarity is regulated by highly conserved cell polarity protein complexes such as the Par3-aPKC-Par6 complex and the CRB3-Pals1-PATJ complex, which are expressed by many different cell types and regulate various aspects of cell polarity. Here we describe a functional interaction of VE-cadherin with the cell polarity protein Pals1. Pals  ...[more]

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